The Adult Immune Response Study

Probiotic supplement consumption alters cytokine production from peripheral blood mononuclear cells: a preliminary study using healthy individuals

Aim

This study examined the effect of a 12-week Lab4 probiotic and fructooligosaccharide supplementation on ex-vivo pro-inflammatory and anti-inflammatory cytokine production by peripheral blood mononuclear cells extracted from healthy volunteers.

Method

  • 20 healthy volunteers received a supplement comprising 25 billion Lab4 probiotics and 2g of fructooligosaccharide (FOS) daily for 12 weeks.
  • Blood samples were collected at the start, 6 and 12 weeks.
  • Peripheral blood mononuclear cells (PBMCs) were isolated from the blood samples and cultured ex-vivo in the absence (‘resting’) or presence (challenge) of lipopolysaccharide (bacterial toxin).
  • Results

    In the ‘resting’ immune state:
    • Significant enhancement of production of the anti-inflammatory cytokines IL-10 and TGF-β over the 12 week period (**P<0.0001)

    Fold change in IL-10 level

    Fold change in IL-1β level - part of the Adult Immune Response study
    Fold change in IL-1β level - part of the Adult Immune Response study

    Fold change in TGF-β level

    Fold change in IL-1β level - part of the Adult Immune Response study
    Fold change in IL-1β level - part of the Adult Immune Response study
    In the ‘resting’ immune state during challenge with bacterial toxin: :
    • Significant decrease in production of the pro-inflammatory cytokines IL-6 and IL-1β(*P<0.01,**P<0.0001)

    Fold change in IL-6 level

    Fold change in IL-1β level - part of the Adult Immune Response study
    Fold change in IL-1β level - part of the Adult Immune Response study

    Fold change in IL-1β level

    Fold change in IL-1β level - part of the Adult Immune Response study
    Fold change in IL-1β level - part of the Adult Immune Response study

    Conclusion

    The consumption of Lab4 probiotics in combination with FOS alters ex vivo cytokine production demonstrating the potential immunomodulatory benefits.

    Reference

    Hepburn NJ et al. 2013. Probiotic supplement consumption alters cytokine production from peripheral blood mononuclear cells: a preliminary study using healthy individuals.
    Beneficial Microbes  4(4): 313-17